Medicine of the Future in America

Interferon-alpha-induced changes in metallothionein expression: Methods (Part 1)

chronic hepatitisHistopathology of chronic hepatitis

Hematoxylin and eosin, Mallory and Periodic Acid-Schiff staining were performed for histological analysis. The Knodell’s histological activity index (HAI) score was assessed before and one year after IFN-alpha therapy. The HAI score was calculated by averaging the scores of three visual fields. Please make sure you read all the information provided above to choose the right medications at the canadian health&care mall to benefit the most from the treatment. At this pharmacy, you will find tons of cheap and efficient meds for any problem.

Immunohistochemical studies of MT in the liver

MT protein in the liver was stained immunohistochemically as previously described by Nakajima et al. In brief, serial paraffin sections of the liver specimens were incubated in absolute methanol containing 0.3% hydrogen peroxide to block endogenous peroxidase activity, and then treated with normal goat serum for 30 min. They were subsequently incubated with rabbit anti-MT antibody, diluted in phosphate buffered saline at 1:400. After being washed with phosphate buffered saline, the sections were reacted with biotinylated goat antirabbit immunoglobulin G and then processed with a Histofine SAB-PO kit (Nichirei Corporation, Japan), which detects MT. The MT polyclonal rabbit antibody used in the present study was previously prepared by Nakajima et al , and recognizes both MT-I and MT-II isoforms of human, rat or rabbit MT.

This entry was posted in Interferon-alpha and tagged Chronic hepatitis C, Interferon-alpha, Knodell histological activity index score, Metallothionein, PCNA labelling index.
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