Medicine of the Future in America

Interferon-alpha-induced changes in metallothionein expression: Discussion (Part 3)

IFN-alpha and -beta are well known for inducing MT-II genes in cultured cells , but little is known on expression patterns of MT isoforms in the liver of HCV-infected patients who have received IFN-alpha therapy. To explore changes in hepatic MT isoform expression resulting from the influence of IFN-alpha, we stained for MT-I and MT-II mRNA in HCV-infected patients both pre- and post-therapy. Because MT-II mRNA increased in correspondence with an increase in PCNA labelling index before therapy, MT-II may be involved in cell proliferation in liver that has been damaged by continuous HCV infection. In addition, MT-II mRNA after therapy correlated positively with HAI scores. The expression of MT-II mRNA before therapy tended was higher than that of MT-I mRNA. On the other hand, MT-I mRNA correlated with neither HAI score nor PCNA index. These data suggest that it is predominantly the MT-II isoform, rather than the MT-I isoform, which is involved in the pathogenesis of HCV infection. You will always be given ventolin 100 mcg and will enjoy paying less money.

Carrera et al demonstrated that hepatic MT levels are a biological factor associated with the severity of HCV infection; however, our data using immunohistochemical staining for MT could not confirm their hypothesis. Further study using quantitative assays for MT protein is necessary to explore the association of MT with the pathogenesis of HCV infection.

This entry was posted in Interferon-alpha and tagged Chronic hepatitis C, Interferon-alpha, Knodell histological activity index score, Metallothionein, PCNA labelling index.
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